Purification and Characterization of a Versatile Peroxidase from Edible Mushroom <i>Pleurotus eryngii</i>

陈敏; 姚善泾; 张虹; 梁新乐

doi:10.1016/S1004-9541(09)60134-8

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Purification and Characterization of a Versatile Peroxidase from Edible Mushroom Pleurotus eryngii

Updated：2026-01-06

- Purification and Characterization of a Versatile Peroxidase from Edible Mushroom Pleurotus eryngii
- Chinese Journal of Chemical Engineering Vol. 18, Issue 5, Pages: 824-829(2010)
- Affiliations：
  
  1. Department of Chemical and Biochemical Engineering, Zhejiang University,Hangzhou,China,310027
  2. Department of Biotechnology Engineering, Zhejiang Gongshang University,Hangzhou,China,310012
- Author bio：
- Funds：
- DOI：10.1016/S1004-9541(09)60134-8
  CLC：
- Published：2010
- Accepted：
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[J]. , 2010, 18(5): 824-829.
DOI：

[J]. , 2010, 18(5): 824-829. DOI： 10.1016/S1004-9541(09)60134-8.

摘要

Abstract

A versatile peroxidase (VP

-Peco60-7

) was generated and purified from the liquid culture of Pleurotus eryngii. The purification

procedure included

ammonium sulfate precipitation

ion exchange chromatography

and gel chromatography. The molecular weight and isoelectric point (pI) of VP

-Peco60-7

were determined to be approxi-mately 40 kDa and 4.1

respectively. By N-terminal sequence determination and peptide mapping analysis

-Peco60-7

was found to be similar to the versatile peroxidase isoenzyme VPL1

which was previously isolated from liquid cultures of the same species. However

the molecular weight and pI of VP

-Peco60-7

were different from those of versatile peroxidases of liquid cultures

implying that the VP

-Peco60-7

in this study is of a novel type. With 2

2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) as a substrate

the maximal enzyme activity was obtained at 50℃ and pH 3.0. The catalysis of ABTS by VP

-Peco60-7

was expressed by the Michaelis-Menten equa-tion. At 50℃ and pH 3.0

the maximum velocity (

max

) was 188.68 U·mg

-1

and the michaelis constant (

) was 203.09 μmol·L

-1

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Purification and Characterization of a Versatile Peroxidase from Edible Mushroom Pleurotus eryngii

DOI：10.1016/S1004-9541(09)60134-8

摘要

Abstract

关键词

Keywords

references