New Development of Reverse Micelles and Applications in Protein Separation and Refolding

刘杨; 董晓燕; 孙彦

doi:10.1016/S1004-9541(09)60022-7

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New Development of Reverse Micelles and Applications in Protein Separation and Refolding

Updated：2026-01-06

- New Development of Reverse Micelles and Applications in Protein Separation and Refolding
- Chinese Journal of Chemical Engineering Vol. 16, Issue 6, Pages: 949-955(2008)
- Affiliations：
  
  Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University,Tianjin,China,300072
- Author bio：
- Funds：
- DOI：10.1016/S1004-9541(09)60022-7
  CLC：
- Published：2008
- Accepted：
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LIU Yang, DONG Xiaoyan, SUN Yan. New Development of Reverse Micelles and Applications in Protein Separation and Refolding[J]. , 2008, 16(6): 949-955.
DOI：

LIU Yang, DONG Xiaoyan, SUN Yan. New Development of Reverse Micelles and Applications in Protein Separation and Refolding[J]. , 2008, 16(6): 949-955. DOI： 10.1016/S1004-9541(09)60022-7.

摘要

Abstract

Reverse micelles bring mild and effective microenvironments in organic solvent that contain biomolecules

which have attracted immense attention for application in the isolation of proteins

protein refolding

and enzymatic reaction.In this review

the application of reverse micelles for protein separation and refolding has been briefly summarized and various reverse micellar systems composed of different surfactants

including ionic

nonionic

mixed

and affinity-based reverse micelles

have been highlighted.It illustrates especially the potential application of the novel affinity-based reverse micelles consisting of biocompatible surfactant coupled with affinity ligands.Moreover

the importance to develop universal affinity-based reverse micelles for protein separation and refolding in the downstream processing of biotechnology has been pointed out.

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