Two-step Enzymatic Synthesis of Guanine Arabinoside

魏晓琨; 丁庆豹; 欧伶; 张鹭; 王昌禄

doi:10.1016/S1004-9541(09)60019-7

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Two-step Enzymatic Synthesis of Guanine Arabinoside

Updated：2026-01-06

- Two-step Enzymatic Synthesis of Guanine Arabinoside
- Chinese Journal of Chemical Engineering Vol. 16, Issue 6, Pages: 934-937(2008)
- Affiliations：
  
  1. State Key Lab of Bioreactor Engineering, East China University of Science and Technology,Shanghai,China,200237
  2. Tianjin University of Science and Technology,Tianjin,China,300222
  3. Shanghai Weiping Bioscience and Biotechnology Co., Ltd.,Shanghai,China,200237
- Author bio：
- Funds：
- DOI：10.1016/S1004-9541(09)60019-7
  CLC：
- Published：2008
- Accepted：
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WEI Xiaokun, DING Qingbao, OU Lin, ZHANG Lu, WANG Changlu. Two-step Enzymatic Synthesis of Guanine Arabinoside[J]. , 2008, 16(6): 934-937.
DOI：

WEI Xiaokun, DING Qingbao, OU Lin, ZHANG Lu, WANG Changlu. Two-step Enzymatic Synthesis of Guanine Arabinoside[J]. , 2008, 16(6): 934-937. DOI： 10.1016/S1004-9541(09)60019-7.

摘要

Abstract

The chemical synthesis of Guanine arabinoside(ara-G)is extremely complex

time-consuming

and seriously polluted.A two-step enzymatic synthesis process was developed to acquire ara-G easily.2

6-Diaminopurine arabinoside(ara-DA)was first synthesized with purine nucleoside phosphorylase and pyrimidine nucleoside phosphorylase produced by

Enterobacter aerogenes

DGW-07.The conversion yield of ara-DA could reach above 90% when the reaction liquid contained 30 mmol·L

-1

uracil arabinoside as arabinose donor

10 mmol·L

-1

6-diaminopurine as arabinose acceptor in pH7.0 20 mmol·L

-1

phosphate buffer

and reacted at 60℃ for 48h.Then

ara-DA was effectively transformed into ara-G with adenylate deaminase produced by

Aspergillus oryzae

DAW-01.The total process had no complex separation and purification.

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