Antioxidant Activity of Isothiocyanate Extracts from Broccoli

袁海娜; 姚善泾; 尤玉如; 肖功年; 尤琦

doi:10.1016/S1004-9541(08)60358-4

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Antioxidant Activity of Isothiocyanate Extracts from Broccoli

Updated：2026-01-06

- Antioxidant Activity of Isothiocyanate Extracts from Broccoli
- Chinese Journal of Chemical Engineering Vol. 18, Issue 2, Pages: 312-321(2010)
- Affiliations：
  
  1. Department of Chemical and Biological Engineering, Zhejiang University,Hangzhou,China,310027
  2. School of Biological and Chemical Engineering, Zhejiang University of Science and Technology,Hangzhou,China,310023
  3. Department of Food Science and Human Nutrition, Clemson University, Clemson SC,United States,29634
- Author bio：
- Funds：
- DOI：10.1016/S1004-9541(08)60358-4
  CLC：
- Published：2010
- Accepted：
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YUAN Haina, YAO Shanjing, YOU Yuru, XIAO Gongnian, YOU Qi. Antioxidant Activity of Isothiocyanate Extracts from Broccoli[J]. , 2010, 18(2): 312-321.
DOI：

YUAN Haina, YAO Shanjing, YOU Yuru, XIAO Gongnian, YOU Qi. Antioxidant Activity of Isothiocyanate Extracts from Broccoli[J]. , 2010, 18(2): 312-321. DOI： 10.1016/S1004-9541(08)60358-4.

摘要

Abstract

Isothiocyanates(ITCs) extracts were prepared from fresh broccoli.Their antioxidant properties were evaluated by using the

in vitro

bioassays

including superoxide anion radical(O

)

hydroxyl radical(HO

) and 2

2-diphenyl-1-picrylhydracyl(DPPH·) radical-scavenging methods

lipid peroxidation assay

and reducing power assay.The ITCs extracts exhibited significant dose-dependent antioxidant activities(

0.01) .Its antioxidant-stability was affected by temperature and storage condition

although it was more stable than vitamin C(Vc) .GC-MS iden-tified sulforaphane in the ITCs extracts.The antioxidant activity of sulforaphane was approximately one tenth to one fifth of that of Vc.Sulforaphane was experimented to be one of dominating contributors to antioxidant capacity of ITCs extracts.However

there was no direct antioxidant activity found for benzyl isothiocyanate(BITC)

in vitro

. Therefore

it could be concluded that the general group(-N=C=S) was not the essential part for the antioxidant activity of sulforaphane.It is suggested that the ITCs extracts from broccoli can be developed into a promising food additive for healthy food.

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