Ag-induced Efficient Immobilization of Papain on Silica Spheres

王安明; 王华; 周成; 杜志强; 祝社民; 沈树宝

doi:10.1016/S1004-9541(08)60129-9

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Ag-induced Efficient Immobilization of Papain on Silica Spheres

Updated：2026-01-06

- Ag-induced Efficient Immobilization of Papain on Silica Spheres
- Chinese Journal of Chemical Engineering Vol. 16, Issue 4, Pages: 612-619(2008)
- Affiliations：
  
  1. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology,Nanjing,China,210009
  2. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Materials Science and Engineering, Nanjing University of Technology,Nanjing,China,210009
- Author bio：
- Funds：
- DOI：10.1016/S1004-9541(08)60129-9
  CLC：
- Published：2008
- Accepted：
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WANG Anming, WANG Hua, ZHOU Cheng, DU Zhiqiang, ZHU Shemin, SHEN Shubao. Ag-induced Efficient Immobilization of Papain on Silica Spheres[J]. , 2008, 16(4): 612-619.
DOI：

WANG Anming, WANG Hua, ZHOU Cheng, DU Zhiqiang, ZHU Shemin, SHEN Shubao. Ag-induced Efficient Immobilization of Papain on Silica Spheres[J]. , 2008, 16(4): 612-619. DOI： 10.1016/S1004-9541(08)60129-9.

摘要

Abstract

Complexation and interaction between silver and amino group were applied to induce an efficient immobilization of papain on silica spheres. The silver nanoparticles were deposited on the silica spheres before papain was coupled to the silica spheres. The silica spheres with silver nanoparticles were characterized by high resolution transmission electron microscopy (HR-TEM)

Fournier transform infrared spectroscopy (FT-IR)

and UV-Vis scanning spectrometer. FT-IR spectrum was also used to characterize the immobilized and free papain. Effect of some factors on the activities of the immobilized papain was investigated. It was observed that the coupled yield and relative activity of the papain on Ag/SiO

were 1.17 and 1.86 times of those on the bare SiO

respectively. At an optimum concentration of silver

the observed activity of the immobilized papain was 2.1 times of that on the bare silica. In addition

the maximum specific activity of papain immobilized on Ag/SiO

was 819.9 U·mg

-1

which is slightly lower than that of the free papain

906.2 U·mg

-1

. Stability of the immobilized papain was also examined. The results indicate that the silver nanoparticles successfully induce a fine immobilization of papain.

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