Traditional biodiesel production primarily uses methanol as the acyl acceptor

but its toxicity to lipase increases process complexity and operational difficulty elevate manufacturing costs. This study aimed to explore a new method for enzymatic synthesis of biodiesel with methyl methacrylate (MMA) as acyl acceptor. Meanwhile

a 1

3-position specific lipase Lipozyme RM IM was applied as biocatalyst

which enables simultaneous production of biodiesel (FAMEs) and methacrylate fatty acid glycerides (MFAGs)

via

specific

sn

-1

3 transesterification of MMA with triglyceride. Under the optimal reaction conditions: temperature of 50 ℃

molar ratio of 4:1 for MMA to triglyceride

enzyme dosage of 7.5% (mass)

and an extra water addition of 0.5% (mass); triglyceride conversion rate of 97%

and FAMEs yield of 65% could be obtained. Simultaneously

the multistage short-path distillation and column chromatographic method were combined used for the separation of the mixed products. Finally

the purity of FAME

MFADG

DMFAG

and MMFAG were 98%

97.8%

95.3%

and 81.78%

respectively. In this new approach

MMA demonstrates lower toxicity to lipases

allowing for straightforward addition of all the substrates without complex addition process

and enhancing operational feasibility. Meanwhile

the by-products of MFAGs could be applied as monomers in varnishes and protective coatings

which increased the value of the products. Thus

this investigation providing a

n alternative way to produce biodiesel

and providing a new pathway for the sustainable development of biodiesel.