Efficient synthesis of tyrosol from L-tyrosine <i>via</i> heterologous Ehrlich pathway in <i>Escherichia coli</i>

Xiaobo Ruan; Sheng Zhang; Wei Song; Jia Liu; Xiulai Chen; Liming Liu; Jing Wu

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Efficient synthesis of tyrosol from L-tyrosine via heterologous Ehrlich pathway in Escherichia coli

Full Length Article | Updated：2026-01-06

- Efficient synthesis of tyrosol from L-tyrosine via heterologous Ehrlich pathway in Escherichia coli
- Chinese Journal of Chemical Engineering Vol. 47, Issue 7, Pages: 18-30(2022)
- Affiliations：
  
  1. School of Pharmaceutical Science, Jiangnan University,Wuxi,China,214122
  2. State Key Laboratory of Food Science and Technology, Jiangnan University,Wuxi,China,214122
  3. Tianrui Chemical Co., Ltd, Department of Chemistry,Quzhou,China,324400
- Author bio：
- Funds：
- DOI：
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- Published：2022
- Accepted：
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摘要

Abstract

For the efficient conversion of L-tyrosine (L-Tyr) to tyrosol

which is an aromatic compound widely used in the pharmaceutical and chemical industries

a novel four-enzyme cascade pathway based on the Ehrlich pathway of

Saccharomyces cerevisiae

was designed and reconstructed in

Escherichia coli

. Then

the expression levels of the relevant enzymes were coordinated using a modular approach and gene duplication after the identification of the pyruvate decarboxylase from

Candida tropicalis

(

PDC) as the rate-limiting enzymatic step.

In situ

product removal (ISPR) strategy with XAD4 resins was explored to avoid product inhibition and further improve tyrosol yield. As a result

the titer and conversion rate of tyrosol obtained were 35.7 g·L

-1

and 93.6%

respectively

in a 3-L bioreactor. Results presented here provide a potential enzymatic process for industrial production of tyrosol from cheap amino acids.

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Related Institution

2 School of Biotechnology, Jiangnan University

2 Department of Chemical and Biological Engineering, Zhejiang University

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4 Department of Chemical Engineering, The University of Utah, Salt Lake City 84102, America

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Efficient synthesis of tyrosol from L-tyrosine via heterologous Ehrlich pathway in Escherichia coli

DOI：

摘要

Abstract

关键词

Keywords

references