Microfluidic reactor with immobilized enzyme-from construction to applications: A review

Han Zhang; Yunpeng Bai; Ning Zhu; Jianhe Xu

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Microfluidic reactor with immobilized enzyme-from construction to applications: A review

Biocatalysis and Bioreactor Engineering | Updated：2026-01-06

- Microfluidic reactor with immobilized enzyme-from construction to applications: A review
- Chinese Journal of Chemical Engineering Vol. 29, Issue 2, Pages: 136-145(2021)
- Affiliations：
  
  1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology,Shanghai,China,200237
  2. College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University,Nanjing,China,211800
- Author bio：
- Funds：
- DOI：
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- Published：2021
- Accepted：
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Han Zhang, Yunpeng Bai, Ning Zhu, Jianhe Xu. Microfluidic reactor with immobilized enzyme-from construction to applications: A review[J]. Chinese Journal of Chemical Engineering, 2021, 29(2): 136-145.
DOI：

Han Zhang, Yunpeng Bai, Ning Zhu, Jianhe Xu. Microfluidic reactor with immobilized enzyme-from construction to applications: A review[J]. Chinese Journal of Chemical Engineering, 2021, 29(2): 136-145. DOI：

摘要

Abstract

Microfluidic

as the systems for using microchannel (micron-or sub-micron scale) to process or manipulate microflow

is being widely applied in enzyme biotechnology and biocatalysis. Microfluidic immobilized enzyme reactor (MIER) is a tool with great value for the study of catalytic property and optimal reaction parameter in a flourishing and highly producing manner. In view of its advantages in efficiency

economy

and addressable recognition especially

MIER occupies an important position in the investigation of life science

including molecular biology

bioanalysis and biosensing

biocatalysis

etc

. Immobilization of enzymes can generally improve their stability

and upon most occasions

the immobilized enzyme is endowed with recyclability. In this review

the enzyme immobilization techniques applied in MIER will be discussed

followed by summarizing the novel developments in the field of MIER for biocatalysis

bioconversion and bioanalysis. The preponderances and deficiencies of the current state-of-the-art preparation ways of MIER are peculiarly discussed. In addition

the prospects of its future study are outlined.

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