The production of enantiopure D-lysine from L-lysine by a two-strain coupled system

Sheng Xu; Xiaolu Lu; Xin Wang; Zhen Gao; Kequan Chen; Pingkai Ouyang

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The production of enantiopure D-lysine from L-lysine by a two-strain coupled system

Special Topic: Biocatalysis | Updated：2026-01-06

- The production of enantiopure D-lysine from L-lysine by a two-strain coupled system
- Chinese Journal of Chemical Engineering Vol. 28, Issue 11, Pages: 2832-2836(2020)
- Affiliations：
  
  State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University,Nanjing,China,211816
- Author bio：
- Funds：
- DOI：
  CLC：
- Published：2020
- Accepted：
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Sheng Xu, Xiaolu Lu, Xin Wang, Zhen Gao, Kequan Chen, Pingkai Ouyang. The production of enantiopure D-lysine from L-lysine by a two-strain coupled system[J]. Chinese Journal of Chemical Engineering, 2020, 28(11): 2832-2836.
DOI：

Sheng Xu, Xiaolu Lu, Xin Wang, Zhen Gao, Kequan Chen, Pingkai Ouyang. The production of enantiopure D-lysine from L-lysine by a two-strain coupled system[J]. Chinese Journal of Chemical Engineering, 2020, 28(11): 2832-2836. DOI：

摘要

Abstract

The microbial production of D-lysine to replace chemical approach has gained great interest with the rising concerns over the environment. Here

we employed recombinant

E. coli

strain BL21-LYR with lysine racemase and strain BL-22A-RB-YB with L-lysine monooxygenase and 5-aminovaleramide amidohydrolase to establish a two-strain coupling whole-cell bioconversion system for D-lysine production from L-lysine. To improve the optical purity of D-lysine

the optimal reaction condition for resolution of DL-lysine after the racemization was investigated. The specificity of BL-22A-RB-YB for L-lysine and the effects of reaction condition on bioconversion efficiency of whole-cell were accordingly determined. Under the optimal condition

a maximum 53.5 g·L

-1

Dlysine and 48.2 g·L

-1

5-AVA were obtained with yield of 47.4% and 42.3%

respectively

by the microbial racemization and asymmetric degradation process. The final D-lysine enantiomeric excess was over 99%. Meanwhile

a valuable compound 5-aminovaleric acid was synthesized with the production of D-lysine

indicating the economic feasibility of the two-strain coupling system.

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