Experimental and in silico studies on three hydrophobic charge-induction adsorbents for porcine immunoglobulin purification

Qilei Zhang; Tiantian Zhuang; Hongfei Tong; Hongyin Wang; Dongqiang Lin; Shanjing Yao

Your Location：

Home >

Browse articles >

Experimental and in silico studies on three hydrophobic charge-induction adsorbents for porcine immunoglobulin purification

第25届中国过程控制会议专栏 | Updated：2026-01-06

- Experimental and in silico studies on three hydrophobic charge-induction adsorbents for porcine immunoglobulin purification
- Chinese Journal of Chemical Engineering Vol. 24, Issue 1, Pages: 151-157(2016)
- Affiliations：
  
  Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Department of Chemical and Biological Engineering, Zhejiang University,Hangzhou,China,310027
- Author bio：
- Funds：
- DOI：
  CLC：
- Published：2016
- Accepted：
Scan QR Code
Qilei Zhang, Tiantian Zhuang, Hongfei Tong, Hongyin Wang, Dongqiang Lin, Shanjing Yao. Experimental and in silico studies on three hydrophobic charge-induction adsorbents for porcine immunoglobulin purification[J]. Chin.J.Chem.Eng., 2016, 24(1): 151-157.
DOI：

Qilei Zhang, Tiantian Zhuang, Hongfei Tong, Hongyin Wang, Dongqiang Lin, Shanjing Yao. Experimental and in silico studies on three hydrophobic charge-induction adsorbents for porcine immunoglobulin purification[J]. Chin.J.Chem.Eng., 2016, 24(1): 151-157. DOI：

摘要

Abstract

Three hydrophobic charge-induction adsorbents with functional ligands of 4-mercapto-ethyl-pyridine

2-mercapto-methyl-imidazole or 2-mercapto-benzimidazole were evaluated in the purification of porcine immunoglobulin from porcine blood. Adsorption isothermswere studied under different pH conditions. The adsorbent with 2-mercapto-methyl-imidazole as the ligand showed reasonable adsorption capacity (43.60 mg·g

-1

gel) with great selectivity and it also showed the best elution performance in chromatographic studies. A multi-pH step elution process was proposed for the 2-mercapto-methyl-imidazole adsorbent

and the results showed that high immunoglobulin purity (94.3%) and a yield of 9.8 mg·(ml plasma)

-1

could be achieved under the optimal condition of loading (pH 5.0)-pre-elution (pH 7.0)-elution (pH 3.8). Moreover

molecular simulation was employed to help in analyzing the binding mechanism between the ligands and immunoglobulin

and the results showed that both 2-mercapto-benzimidazole and 2-mercapto-methyl-imidazole ligands were docked on the same pocket (around TYR319 and LEU309) of the Fc fragment of immunoglobulin

with 2-mercaptobenzimidazole showing stronger binding interactions.

关键词

Keywords

references

Views

192

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Unraveling the intricate solvent-catalyst interplay in TS-1 catalyzed benzene hydroxylation: Experimental and molecular simulation insights

Variable pseudo-particle modeling for molecular dynamics simulation

Coarse-grained molecular dynamics simulations on self-assembly of polystyrene-block-poly(2-vinylpyridine)

Design method of extractant for liquid-liquid extraction based on elements and chemical bonds

Study on the evolution of solid–liquid–gas in multi-scale pore methane in tectonic coal

Related Author

Jiang Yingdi

Zhan Wanbin

Yang Chao

Zhang Jing

Xia Changjiu

Qian Gang

Duan Xuezhi

Qiu Tianhao

Related Institution

State Key Laboratory of Chemical Engineering and Low-Carbon Technology, East China University of Science and Technology

Sinopec Research Institute of Petroleum Processing

School of Energy Science and Engineering, Harbin Institute of Technology

State Key Laboratory of Mesoscience and Engineering, Institute of Process Engineering, Chinese Academy of Sciences

School of Chemical Engineering, University of Chinese Academy of Sciences

⁰