Optimizing the Chemical Compositions of Protective Agents for Freeze-drying <i>Bifidobacterium longum</i> BIOMA 5920<sup>*</sup>

杨婵媛; 朱晓丽; 范代娣; 米钰; 骆艳娥; 惠俊峰; 苏然

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Optimizing the Chemical Compositions of Protective Agents for Freeze-drying Bifidobacterium longum BIOMA 5920^*

Updated：2026-01-06

- Optimizing the Chemical Compositions of Protective Agents for Freeze-drying Bifidobacterium longum BIOMA 5920^*
- Chinese Journal of Chemical Engineering Vol. 20, Issue 5, Pages: 930-936(2012)
- Affiliations：
  
  1. Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University,Xi'an,China,710069
  2. Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University,Xi'an,China,710069
  3. Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Urban and Environmental Science, Northwest University,Xi'an,China,710069
- Author bio：
- Funds：
- DOI：
  CLC：
- Published：2012
- Accepted：
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BIOMA 5920
DOI：

BIOMA 5920 DOI：

摘要

Abstract

Freeze drying has a deleterious effect on the viability of microorganisms. In front of this difficulty

the present study adopts response surface methodology to optimize the chemical compositions of protective agents to seek for maximum viability of

Bifidobacterium longum

BIOMA 5920 during freeze-drying. Through the comparative analysis of single protectant

the complex protective agents show better effect on the

Bifidobacterium

viability. Human-like collagen (HLC)

trehalose and glycerol are confirmed as significant factors by Box-Behnken Design. The optimized formula for these three variables is tested as follows: HLC 1.23%

trehalose 11.50% and glycerol 4.65%. Under this formula

the viability is 88.23%

39.67% higher in comparison to the control. The viable count is 1.07×10

cfu·g

-1

greatly exceeding the minimum viable count requirement (10

cfu·g

-1

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Optimizing the Chemical Compositions of Protective Agents for Freeze-drying Bifidobacterium longum BIOMA 5920*

DOI：

摘要

Abstract

关键词

Keywords

references

Optimizing the Chemical Compositions of Protective Agents for Freeze-drying Bifidobacterium longum BIOMA 5920^*