Synthesis of Wax Esters by Lipase-catalyzed Esterification with Immobilized Lipase from <i>Candida</i> sp. 99-125

邓利; 王晓静; 聂开立; 王芳; 刘军峰; 王璞; 谭天伟

Your Location：

Home >

Browse articles >

Synthesis of Wax Esters by Lipase-catalyzed Esterification with Immobilized Lipase from Candida sp. 99-125

Updated：2026-01-06

- Synthesis of Wax Esters by Lipase-catalyzed Esterification with Immobilized Lipase from Candida sp. 99-125
- Chinese Journal of Chemical Engineering Vol. 19, Issue 6, Pages: 978-982(2011)
- Affiliations：
  
  1. College of Life Science and Technology, Beijing Key Laboratory of Bioprocess, Biorefinery Research and Engineering Center, Ministry of Education, Beijing University of Chemical Technology,Beijing,China,100029
  2. College of Chemical Engineering, Beijing University of Chemical Technology,Beijing,China,100029
- Author bio：
- Funds：
- DOI：
  CLC：
- Published：2011
- Accepted：
Scan QR Code
sp. 99-125[J]. , 2011, 19(6): 978-982.
DOI：

sp. 99-125[J]. , 2011, 19(6): 978-982. DOI：

摘要

Abstract

Wax esters were synthesized in a solvent free system catalyzed by immobilized lipase from

Candida

sp. 99-125

with oleic acid and cetyl alcohol. The effects of substrate molar ratio

lipase dosage and water removal were investigated in a 50 ml flask incubated in a thermostatic cultivation cabinet. The optimized conditions were: temperature 40℃

shaking at 170 r·min

-1

acid/alcohol molar ratio 1:0.9

lipase dosage in 10% (by mass) of oleic acid

and open reaction for water removal. As a result

the conversion rate reached 98% for reaction of 8 h. The volume of reactor was scaled up to 1 L three-neck flask. The optimized parameters were: 200 r·min

-1

agitation speed

2.5% (by mass) lipase dosage

others were the same as the parameters described above. The conversion rate reached 95% for reaction of 24 h. The lipase retained 46% conversion rate after reuse for 6

7 batches. The products were purified by removing remained cetyl alcohol and fatty acids with ethanol and saturated sodium carbonate solution

respectively. The purity of the wax ester

cetyl oleate

was 96%. The physical and chemical properties of cetyl oleate were tested and compared with those of jojoba oil. The results show that the product cetyl oleate has great potential to use as the substitute of natural jojoba oil.

关键词

Keywords

references

Views

2816

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Lipase and photodecarboxylase coexpression: A potential strategy for alkane-based biodiesel production from natural triglycerides

Conjugation of a zwitterionic polymer with dimethyl chains to lipase significantly increases the enzyme activity and stability

Fabrication and characterization of epoxylated zwitterionic copolymergrafted silica nanoparticle as a new support for lipase immobilization

Lipase-catalyzed Synthesis of Caffeic Acid Phenethyl Ester in Ionic Liquids：Effect of Specific Ions and Reaction Parameters

Enzyme-catalyzed Synthesis of Vitamin E Succinate Using a Chemically Modified Novozym-435

Related Author

Bi-Shuang Chen

Xin-Jian Yin

Lan Liu

Wen-Li Chen

Jin-Xuan Xie

Xin-Yi Xu

Yong-Yi Zeng

Xiaoyan Dong

Related Institution

School of Marine Sciences, Sun Yat-Sen University

Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai)

Department of Biochemical Engineering, School of Chemical Engineering and Technology and Key Laboratory of Systems Bioengineering and Frontiers Science Center for Synthetic Biology (Ministry of Education), Tianjin University

College of Life Sciences, Yantai University

Department of Biochemical Engineering and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University

⁰

Synthesis of Wax Esters by Lipase-catalyzed Esterification with Immobilized Lipase from Candida sp. 99-125

DOI：

摘要

Abstract

关键词

Keywords

references