Enzymatic Synthesis of Fatty Acid Methyl Esters from Crude Rice Bran Oil with Immobilized Candida sp. 99-125

李政; 邓利; 鲁吉珂; 郭小雷; 杨自信; 谭天伟

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Enzymatic Synthesis of Fatty Acid Methyl Esters from Crude Rice Bran Oil with Immobilized Candida sp. 99-125

Updated：2026-01-06

- Enzymatic Synthesis of Fatty Acid Methyl Esters from Crude Rice Bran Oil with Immobilized Candida sp. 99-125
- Chinese Journal of Chemical Engineering Vol. 18, Issue 5, Pages: 870-875(2010)
- Affiliations：
  
  1. Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology,Beijing,China,100029
  2. School of Textiles, Tianjin Polytechnic University,Tianjin,China,300160
  3. Bioengineering Department, Zhengzhou University,Zhengzhou,China,450001
- Author bio：
- Funds：
- DOI：
  CLC：
- Published：2010
- Accepted：
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LI Zheng, DENG Li, LU Jike, GUO Xiaolei, YANG Zixin, TAN Tianwei. Enzymatic Synthesis of Fatty Acid Methyl Esters from Crude Rice Bran Oil with Immobilized Candida sp. 99-125[J]. , 2010, 18(5): 870-875.
DOI：

LI Zheng, DENG Li, LU Jike, GUO Xiaolei, YANG Zixin, TAN Tianwei. Enzymatic Synthesis of Fatty Acid Methyl Esters from Crude Rice Bran Oil with Immobilized Candida sp. 99-125[J]. , 2010, 18(5): 870-875. DOI：

摘要

Abstract

The non-edible crude rice bran oil was extracted from white rice bran

and then was catalyzed by immobilized lipase for biodiesel production in this study. The effects of water content

oil/methanol molar ratio

temperature

enzyme amount

solvent

number of methanol added times and two-step methanolysis by using Candida sp. 99-125 as catalyst were investigated. The optimal conditions for processing 1 g rice bran oil were:0.2 g immobilized lipase

2 ml n-hexane as solvent

20% water based on the rice bran oil mass

temperature of 40℃ and two-step addition of methanol. As a result

the fatty acid methyl esters yield was 87.4%. The immobilized lipase was proved to be stable when it was used repeatedly for 7 cycles.

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