Polyurethane-decellularized extracellular matrix electrospun composite fibers as urethral scaffold

Piolo Gabriel N. Gervacio; Joseph Rey H. Sta Agueda; Allan N. Soriano; Nathaniel P. Dugos

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Polyurethane-decellularized extracellular matrix electrospun composite fibers as urethral scaffold

Updated：2026-08-24

- Polyurethane-decellularized extracellular matrix electrospun composite fibers as urethral scaffold
- Chinese Journal of Chemical Engineering Vol. 94, Issue 6, Pages: 73-80(2026)
- Affiliations：
  
  Department of Chemical Engineering, De La Salle University, Malate, Manila 1004, Philippines
  Department of Manufacturing Engineering and Management, De La Salle University, Malate, Manila 1004, Philippines
- Author bio：
  
  Corresponding author. E-mail address: nathaniel.dugos@dlsu.edu.ph(N.P. Dugos)
- Funds：
- DOI：
  CLC：
- Received：16 September 2025，
  
  Revised：2025-12-14，
  
  Accepted：13 January 2026，
  
  Online First：07 March 2026，
  
  Published：2026-06
- Accepted：
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Piolo Gabriel N. Gervacio, Joseph Rey H. Sta Agueda, Allan N. Soriano, et al. Polyurethane-decellularized extracellular matrix electrospun composite fibers as urethral scaffold[J]. Chinese Journal of Chemical Engineering, 2026, 94(6): 73-80.
DOI：

Piolo Gabriel N. Gervacio, Joseph Rey H. Sta Agueda, Allan N. Soriano, et al. Polyurethane-decellularized extracellular matrix electrospun composite fibers as urethral scaffold[J]. Chinese Journal of Chemical Engineering, 2026, 94(6): 73-80. DOI：

摘要

Abstract

Scaffolding is less intrusive for damaged urethral tissue regeneration compared to tissue grafting. The study focuses on polyurethane-decellularized extracellular matrix (PU-dECM) scaffolds electrospun produced from varying PU-dECM concentrations and electrospinning voltages for the production of tunable biomimetic scaffolds as an alternative specialized urethral restoration method. Porcine urethra dECM was produced through immersion-sonication at 0.5% sodium dodecyl sulfate (SDS)

followed by solubilization. PU and dECM were dissolved in 7:3 dimethyl sulfoxide: ethanol (DMSO:E

tOH) solvent at ratios of 90:10

85:15

and 80:20 for a total polymer concentration of 9.5%. Following this

electrospinning was conducted at 29-35 kV

3.75 ml·h

- 1

flowrate for 90 min. Viscosity decreased while conductivity increased with increasing dECM concentration. Increasing dECM concentration also decreased the Young's moduli

and the concentration-voltage interaction introduced a significant effect on the Young's moduli. The treatments of 80:20-29 kV

90:10-29 kV

and 90:10-32 kV (PU:dECM ratioelectrospinning voltage) were predicted to have Young's moduli close to that of the human urethra at (0.034 ± 0.01) MPa and were selected as candidate fibers for characterization. Fibers with random orientation and diameters of 1.05-1.41

1.34-1.51 and 1.30-1.45 μm respectively were produced. Coating of dECM is observed from FTIR spectra. For urethral scaffold applications

the 90:10@29 kV fibers reported the best hydrophilicity at 46.34°±24.07°.

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Keywords

references

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