Efficient leukocyte removal and enhanced biocompatibility using PVDF membranes prepared by vapor-induced phase separation

Jin Ziqi; Yao Shuang; Li Liang; Sun Siyuan; Zhou Yue; Zhou Jie; Wang Zhaohui; Cui Zhaoliang

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Efficient leukocyte removal and enhanced biocompatibility using PVDF membranes prepared by vapor-induced phase separation

Updated：2026-03-13

- Efficient leukocyte removal and enhanced biocompatibility using PVDF membranes prepared by vapor-induced phase separation
- Chinese Journal of Chemical Engineering Vol. 89, Issue 1, Pages: 1-12(2026)
- Affiliations：
  
  State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemical Engineering, Nanjing Tech University, Nanjing 210009, China
  National Engineering Research Center for Special Separation Membrane, Nanjing Tech University, Nanjing 210009, China
  Jiangsu IKMO Membrane High-Technology Co., Ltd, Suzhou 215300, China
  Suzhou Future Membrane Technology Innovation Center, Nanjing Tech University, Suzhou 215300, China
- Author bio：
  
  Corresponding author. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemical Engineering, Nanjing Tech University, Nanjing 210009, China. E-mail address: zcui@njtech.edu.cn(Z. Cui).
- Funds：
- DOI：
  CLC：
- Received：20 May 2025，
  
  Revised：2025-08-14，
  
  Accepted：15 August 2025，
  
  Online First：14 October 2025，
  
  Published：2026-01
- Accepted：
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Jin Ziqi, Yao Shuang, Li Liang, et al. Efficient leukocyte removal and enhanced biocompatibility using PVDF membranes prepared by vapor-induced phase separation[J]. Chinese Journal of Chemical Engineering, 2026, 89(1): 1-12.
DOI：

Jin Ziqi, Yao Shuang, Li Liang, et al. Efficient leukocyte removal and enhanced biocompatibility using PVDF membranes prepared by vapor-induced phase separation[J]. Chinese Journal of Chemical Engineering, 2026, 89(1): 1-12. DOI：

摘要

Abstract

To develop an efficient filter for removing white blood cells from whole blood

hydrophilic large-pore blended membranes of poly(vinylidene fluoride) (PVDF)

polyvinyl pyrrolidone and polyethylene glycol

with good biocompatibility

were prepared using the process of vapor-induced phase separation at various PVDF concentrations. The results demonstrated that at a PVDF mass concentration of 14%

the membrane had increased surface roughness

significantly enhanced hydrophilicity and wettability

and a wetting time of 8 s. The surface roughness of the membrane was also reduced to 31.637 nm. Furthermore

hemolysis rate and protein adsorption tests indicated that the blended membranes possessed excellent biocompatibility. They were reduced to 2.48% and 34.44 μg·cm

- 2

respectively. The pore size of the fabricated membrane was relatively large

which reached approximately 8 μm respectively

satisfying the filtration requirements. Lastly

the effects of different temperatures and multi-layered filters on leukocyte remov

al and the retention of red blood cells and platelets from whole blood were evaluated. The results revealed that the leukocyte removal rate was highest at 4

◦

C and with three membrane layers

the leukocyte removal rate was highest

reaching 98.36%

while the RBC and platelet content remained nearly unchanged compared with the original blood. This study provides a new approach for blood cell separation that is expected to play a significant role in medical fields such as blood transfusion demonstrating great potential for application and innovation.

关键词

Keywords

references

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