Abstract: Egg yolk phosphatidylcholine (EYPC) is being widely used in food and pharmaceutical industries nowadays, because of its surface activity, pharmaceutical usefulness and so on. Common determination methods of phospholipids were based on the American Oil Chemists' Society (AOCS) Official Method Ja7b-91 which uses n-hexane/2-propanol/acetate buffer as the mobile phase. To achieve the desired results, gradient elution or buffer solution were needed. This makes the detection process more complicated. Moreover, water or buffer solution could significantly affect the silica gel column both on its lifespan and the separation efficiency. In this study, we used different mobile phase and different detector to simplify EYPC analyzing process and avoided using water within the mobile phase. The optimized HPLC operating conditions are shown as follow: pure methanol as a mobile phase, 1.0 ml/min flow rate，silica gel column (250 mm 】 4.6 mm, 5 レm, Inertsil GL⑩), column temperature 30∩ and low temperature evaporative light scattering detector was used. Under this condition, the standard curve linear relative coefficient is 0.998 and the recovery was in the range of 96.83%-101.58% with a relative standard deviation of 1.79% (n=6).
周婧 袁其朋 胡仙妹 王田心. HPLC-ELSD法分析蛋黄磷脂酰胆碱 [J]. .
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