• •    



  1. 1. 北京化工大学 生命科学与技术学院
    2. 北京化工大学
  • 收稿日期:2010-11-23 修回日期:2012-02-15 发布日期:2012-07-06
  • 通讯作者: 袁其朋

HPLC Analysis of Egg Yolk Phosphatidylcholine by Evaporative Light Scattering Detector

  • Received:2010-11-23 Revised:2012-02-15 Published:2012-07-06


关键词: 蛋黄磷脂酰胆碱, 高效液相色谱, 蒸发光散射检测器

Abstract: Egg yolk phosphatidylcholine (EYPC) is being widely used in food and pharmaceutical industries nowadays, because of its surface activity, pharmaceutical usefulness and so on. Common determination methods of phospholipids were based on the American Oil Chemists' Society (AOCS) Official Method Ja7b-91 which uses n-hexane/2-propanol/acetate buffer as the mobile phase. To achieve the desired results, gradient elution or buffer solution were needed. This makes the detection process more complicated. Moreover, water or buffer solution could significantly affect the silica gel column both on its lifespan and the separation efficiency. In this study, we used different mobile phase and different detector to simplify EYPC analyzing process and avoided using water within the mobile phase. The optimized HPLC operating conditions are shown as follow: pure methanol as a mobile phase, 1.0 ml/min flow rate,silica gel column (250 mm 】 4.6 mm, 5 レm, Inertsil GL⑩), column temperature 30∩ and low temperature evaporative light scattering detector was used. Under this condition, the standard curve linear relative coefficient is 0.998 and the recovery was in the range of 96.83%-101.58% with a relative standard deviation of 1.79% (n=6).